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KMID : 0616120090400030238
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Korean Journal of Pharmacognosy
2009 Volume.40 No. 3 p.238 ~ p.243
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Inhibitory Effects of 23 Korean Local Plant Extracts on Aldo-keto reductase family 1 B10 (AKR 1 B10)
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Lee Joo-Young
Song Dae-Geun
Jung Sang-Hoon
Kim Jong-Hwan
Ahn Soo-Young
Nho Chu-Won
Pan Cheol-Ho
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Abstract
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We examined ethanol extracts prepared from 23 Korean local plants obtained in Pyeongchang, Gangwon-do for their inhibitory effects on recombinant human AKR 1 B10 (rhAKR1B10) in vitro. To do this, rhAKR1B10 was first expressed in E. coli as a biological active form and purified by using Ni-affinity chromatography followed by gel permeation chromatography. Then, rhAKR1B10 was used for screening out 23 Korean local plant extracts having an inhibitory activity against itself. Among them, six extracts showed more than 50% inhibition of rhAKR1B10 activity at the concentration of 10㎍/ml. Especially, the extracts of Ligularia fischeri var. spiciformis Nakai and Rhus trichocarpa Miq. were the most potent because their IC50 values were 2.94 and 2.00㎍/ml, respectively.
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KEYWORD
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Aldo-keto reductase family 1 B10, aldose reductase-like 1, anti-cancer, Ligularia fischeri, Rhus trichocarpa
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